Papers 2010-present
Jevon, D, Deng K, Hallahan, N, Kumar K, Tong J, Gan, WJ, Tran, C, Bilek, MM, Thorn P (2022) Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose dependent insulin secretion. eLife in press link here
Koneshamoorthy, A., Seniveratne-Epa, D., Calder, G., Sawyer, M., Kay, T., Farrell, S., Loudovaris, T., Mariana, L., McCarthy, D., Lyu, R., Liu, X., Thorn, P., Tong, J., Chin, L. K., Zacharin, M., Trainer, A., Taylor, S., MacIsaac, R. J., Sachithanandan, N., Thomas, H. E., … Krishnamurthy, B. (2022). Case Report: Hypoglycemia Due to a Novel Activating Glucokinase Variant in an Adult - a Molecular Approach. Frontiers in endocrinology, 13, 842937. link here
K Deng & Thorn P (2022) Presynatpic like mechanisms and the control of insulin secretion in pancreatic β cells. Cell Calcium 102585 link here
Eriksson M, Litwak S, Yun Y, Stanley W, Thorn P, Ahlgren U, Gurzov EN (2021) Insulin-binding peptide probes provide a novel strategy for pancreatic b-cell imaging. Molecular Pharmaceutics 18 4428-4436 link here
Yau B, Naghiloo S, Diaz-Vegas A, Carr AV, Van Gerwen J, Needham EJ, Jevon D, Chen SY,Hoehn KL, Brandon AE, Macia L, Cooney GJ, Shortreed MR, Smith LM, Keller MP, Thorn P, Larance M, James DE, Kebede MA. (2021) Proteomic pathways to metabolic disease and type 2 diabetes in the pancreatic islet. iScience 24 103099 link here
Tran C, Hallahan N, Kosobrodova E, Tong J, Thorn P, Bilek M. (2021) Plasma Surface Engineering to Biofunctionalise Polymers for β-Cell Adhesion. Coatings. 11(9):1085 link here
Burganova G, Bridges C, Thorn P, Landsman L (2021) The role of vascular cells in pancreatic beta cell function. Frontiers in Endocrinology,12:667170 link here
Cottle L, Gilroy I, Deng K, Loudovaris T, Thomas HE, Gill AJ, Samra J, Kebede MA, Kim J and Thorn P (2021) Machine Learning Algorithms, Applied to Intact Islets of Langerhans, Demonstrate Significantly Enhanced Insulin Staining at the Capillary Interface of Human Pancreatic β Cells. Metabolites 11(6):363. link here
Cottle L., Gan WJ., Gilroy I., Samra JS., Gill AJ., Loudovaris T., Thomas HE., Hawthorne WJ., Kebede MA. And Thorn P (2021) Structural and functional polarisation of human pancreatic beta cells in islets from organ donors with and without type 2 diabetes. Diabetologia 68:618-629. link here
Tan R, Hallahan N, Kosobrodova E, Michael P, Wei F, Santos M, Lam YT, Chan AHP, Xiao Y, Bilek MM, Thorn P, Wise SG. (2020) Bioactivation of encapsulation membrane reduced fibrosis and enhances cell survival. Applied Materials & Interfaces.12, 51, 56908–56923 link here
Singh R., Cottle L., Loudovaris T., Xiao D., Yang P., Thomas HE., Kebede MA. And Thorn P (2021) Enhanced Structure and Function of Human Pluripotent Stem Cell-Derived Beta-Cells Cultured on Extracellular Matrix. Stem Cells Translational Medicine. 10:492-505. link here
Yau, B., Hays, L., Liang, C., Laybutt, D.R., Thomas, H.E., Gunton, J.E., Williams, L., Hawthorne, W.J., Thorn P., Rhodes, C.J.,and Kebede M.A. (2020) A fluorescent timer reporter enables sorting of insulin secretory granules by age. J Biol Chem 295(27):8901-8911 link here
Ma, W., Chang, J., Tong, J., Ho, U., Yau B., Kebede, MA. And Thorn, P. (2020) Arp2/3 nucleates F-actin coating of fusing insulin granules in pancreatic beta cells to control insulin secretion. J Cell Sci 133 jcs236794 link here
Ursino, GM., Fu, Y, Cottle, DL., Mukhamedova, N., Jones, LK., Low, H., Tham, MS., Gan, WJ., Mellet, NA., Das, PP., Weir, JM., Ditiatkovoski, M., Fynch, S., Thorn, P., Thomas, HE., Meikle, PJ., Parkington, HC., Smyth IM and Sviridov D. (2020) ABCA12 regulates insulin secretion from beta-cells EMBO Reports 21:e48692 link here
Lammert E and Thorn P (2020) The role of the islet niche on the structure and function of beta cells. Journal of Molecular Biology 432, 1407-1418 link here
Gan WJ, Do OH, Cottle L, Ma W, Kosobrodova E, Cooper-White J, Bilek M, Thorn P (2018) Local integrin activation in pancreatic beta cells targets insulin secretion to the vasculature. Cell Reports 24, 2819-2826 link here
Dolai S, Liang T, Orabi A, Xie L, Holmyard D, Javed T, Fernandez N, Xie H, Cattral M, Thurmond D, Thorn P, Gaisano HY (2018) Munc18c depletion attenuates caerulein hyperstimulation induced pancreatitis. J Biol Chem 293, 2510-2522 link here
Dolai S, Liang T, Orabi A, Holmyard D, Xie L, Greitzer-Antes D, Kang Y, Xie H, Javed T, Lam P, Rubin D, Thorn P, and Gaisano HY. (2018) Pancreatitis-induced depletion of syntaxin-2 deregulates autophagy and enhances basolateral exocytosis. Gastroenterology 154: 1805-1821 link here
Kosobrodova E, Wan JG, Kondyurin A., Thorn P* and Bilek MMM* (2018) *joint senior author. Improved multi-protein micro-contact printing on plasma immersion ion implanted polystyrene. ACS Applied Materials and Interfaces. 10: 227-237 link here
Liang T, Dolai S, Xie L, Winter E, Orabi AI, Karimian N, Cosen-Binker LI, Huang YC, Thorn P, Cattral MS, Gaisano HY (2017) Ex vivo human pancreatic slice preparations offer a valuable model for studying pancreatic exocrine biology. J Biol Chem 292: 5957-5969 link here
Gan WJ, Zavortink M, Ludick C, Templin R, Webb R, Webb R, Poronnik P, Parton RG, Gaisano HY, Shewan AM, and Thorn P (2017) Cell polarity defines three distinct domains in pancreatic beta cells. Journal of Cell Science, 130: 143 -51
Han Y, Shewan AM and Thorn P (2016) HCO3- transport through anoctamin/transmembrane protein ANO1/TMEM16A, in pancreatic acinar cells, regulates luminal pH. J Biol Chem 291: 20345-20352
Do OH, Gunton J, Gaisano HY and Thorn P (2016) Changes in beta cell function occur in prediabetes and early disease in the Leprdb mouse model of diabetes. Diabetologia, 59: 1222-1230
Yu M, Niu Y, Zhang Y, Zhang H, Yang Y, Taran E, Jambhrunkar S, Gu W, Thorn P, and Yu C(2016). Size-dependent gene delivery of amine modified silica nanoparticles. Nano Research, 9, 291-305
Thorn P and Gaisano HY (2015) Cell-to-cell communication and the regulation of pancreatic function. Pancreas 44, 1174-1175
Do OH, Low JT and Thorn P (2015) Insulin secretion from beta cells within intact islets; location matters. Clinical & Experimental Pharmacology & Physiology, 42, 406-414
Do OH, Low JT and Thorn P (2015) Leprdb mouse model of type 2 diabetes: pancreatic islet isolation and live-cell 2-photon imaging of intact islets. Journal of Visualized Experiments Issue 99; doi: 10.3791/52632
Maisonneuve B, Roux D, Thorn P Cooper-White J, (2015) Effects of Synthetic Biomacromolecule Addition on the Flow Behaviour of Concentrated Mesenchymal Cell Suspensions. Biomacromolecules 16, 275-283
Low JT, Zavortink M, Mitchell JM, Gan WJ, Schwiening CJ, Gaisano HY, and Thorn P (2014) Insulin secretion from beta cells in intact mouse islets is targeted towards the vasculature. Diabetologia 57: 1655-1663
Do OH, Low JT, Gaisano HY and Thorn P (2014) The secretory deficit in db/db islets is mainly due to a loss of responding beta cells. Diabetologia 57: 1400-1409
Yu M, Karmakar S, Yang J, Zhang H, Yang Y, Thorn P and Yu C. (2014) Facile synthesis of ultra-small hybrid silica spheres for enhanced penetration of 3D glioma spheroids. Chem Comm 50, 1527-1529.
Meihua Y, Yuting N, Yannan Y, Sandy BH, Jie Y, Thorn P, Xiaodan H and Yu C. (2014) A novel approach to prepare polyethyleneimine functionalized silica-based spheres with small size for siRNA delivery. ACS Applied Materials and Interfaces. 6: 15626-15631
Thorn P. (2014) Measurement of dynamic F-actin changes during exocytosis. Methods in Molecular Biology. 1174:423-431
Jiun T. Low , Justin M. Mitchell, Oanh H. Do, Jacinda Bax, Alicia Rawlings, Michael Zavortink, Garry Morgan, Robert G. Parton, Herbert Y. Gaisano and Peter Thorn (2013). Glucose principally regulates insulin secretion in islets by controlling the numbers of granule fusion events per cell. Diabetologia 56: 2629-2637
Maisonneuve B, Roux, D.C.D. Thorn, P. & Cooper-White, J.J. (2013) Effects of cell density and biomacromolecule addition on the flow behaviour of concentrated mesenchymal cell suspensions. Biomacromolecules 14 12: 4388-4397
Meihua Yu., Gu W., Jambhrunkar S., Thorn P., Chen J., Yu C . (2013) Hyaluronic Acid Modified Mesoporous Silica Nanoparticles for Targeted Drug Delivery to CD44-overexpressing Cancer Cells. Nanoscale 5: 178-183.
Thorn,P. Measuring calcium signals and exocytosis in tissues. (2012) Biochemica et Biophysica Acta – General Subjects 1820(8):1179-84.
Thorn, P. and Gaisano H.Y. (2012) Molecular control of compound exocytosis: a key role for VAMP8. Communicative and integrative Biology 5, issue 1, 61-63.
Jang Y., Soekmadji C., Mitchell, J.M, Thomas, W.G. and Thorn, P. (2012) Real-time measurement of F-actin remodelling during exocytosis using Lifeact-EGFP transgenic animals. PLOS One 7(7): e39815.
Meihua Yu., Zhou, L., Zhang, J., Yuan, P., Thorn, P., Gu, W., Yu, CY. (2012) A Simple Approach to Prepare Monodisperse Mesoporous Silica Nanospheres with Adjustable Sizes Journal of Colloidal and Interface Science 376(1):67-75.
Bugarcic, A., Zimbardi, K., Macaranas J. and Thorn P. (2012) An inquiry-based practical for a large, foundation-level undergraduate laboratory that enhances student understanding of basic cellular concepts and scientific experimental design. Biochemistry and Molecular Biology Education 40(3):174-80.
Mangold S, Wu SK, Norwood SJ, Collins BM, Hamilton NA, Thorn P, Yap AS (2011) Hepatocyte growth factor acutely perturbs actin filament anchorage at the epithelial zonula adherens. Current Biology 21(6):503-7.
Jang, Y. & Thorn, P. (2011) Visualization of exocytosis in pancreatic acinar cells by fluorescence microscopy. The Pancreapedia: Exocrine Pancreas Knowledge Base, DOI: 10.3998/panc.2011.15
Behrendorff, N., Dolai, S., Hong, W., Gaisano, H.Y. & Thorn, P. (2011) Vesicle-associated Membrane Protein 8 (VAMP8) Is a SNARE (Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor) Selectively Required for Sequential Granule-to-granule Fusion. Journal of Biological Chemistry 286, 29627-29634.
Behrendorff N, Floetenmeyer, M, Schwiening C & Thorn P (2010) Protons, co-released during secretion, are a negative feedback extracellular messenger and contribute to the early tissue damage in secretagogue-induced pancreatitis Gastroenterology 139, 1711-1720.
Soekmadji, C. & Thorn, P. (2010) Secretory control: evidence for agonist-regulation of post-fusion behaviour. Clinical and Experimental Pharmacology and Physiology 37, 218-221.
Low J.T., Shukla A., Behrendorff N. & Thorn, P. (2010) Exocytosis, dependent on calcium release from calcium stores, is regulated by calcium microdomains. Journal of Cell Science 123, 3201-3208
Low J.T, Shukla A. & Thorn, P. (2010) Pancreatic acinar cell: new insights into the control of secretion. The International Journal of Biochemistry & Cell Biology 42, 1586–1589